Influence of Serial Subcultivation on the Immunoregulation of Retinal Pigment Epithelialium Cells in vitro / 医学研究杂志

ABSTRACT

Objective To investigate the influence of serial subcultivation on the immunoregulation of retinal pigment epithelialium cells in vitro.Methods Co-culture-systems of HRPE cells with lymphocytes were established in vitro.The expression of Fas on different passages of HRPE cells and the induction of apoptosis on lymphocytes were observed by flow cytometry. Results When co-cultured with lymphocytes,the expression of Fas was tapering and the duration of culture in vitro was significantly correlated with the expression of Fas(the rate of positive cell r=-0.859,P0.05).Conclusion HRPE cells beyond 3 passages showed a 1ow capability of immunoregulation and were not suitable for seeds cells for HRPE transplantation.