DETERMINATION OF FOLIC ACID IN INFANT FORMULA MILK POWDER BY SOLID-PHASE EXTRACTION-REVERSED PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY / 营养学报

ABSTRACT

Objective A quick and sensitive method was developed for determination of folic acid (FA) in infant formula milk powder. Method Perchloric aicd was added into infant formula milk powder to extract FA in ultrasonic wave condition. After solid-phase extraction (SPE) with C18 material as plug and reaction with potassium permanganate, FA was determined by reversed phase high-performance liquid chromatography (RP-HPLC). pH 5.0 and 0.1 mol/L sodium dihydrogen phosphate were used as mobile phase. the flow rate was 1 ml/min. BDS C18 was used as separating column and column temperature was 30 ℃. Results The linear range of the method was 0.005-1.5 ?g/ml. The average recovery was 91.7%- 98.3%. The relative standard deviation (RSD) was 2.5%. The limit of detection was 0.005 ?g/ml, and the limit of quantification was 0.017 ?g/ml. Conclusion The method has following advantages: simple, precise, accurate and easy to practice.