Effects of stretch load on the ultramicrostructure and c-kit gene expression of the bladder ICCs in rats / 第三军医大学学报

ABSTRACT

Objective To investigate the effects of stretch load on the ultramicrostructure and c-kit expression of the bladder interstitial cells of cajal (ICCs) in rats. Methods Rat model with detrusor overactivity due to partial outflow obstruction with bladder neck ligation were established. The effects of stretch load on the changes of ultramicrostructure of the bladder ICCs were observed by scanning electron microscopy. The changes in c-kit gene expression of ICCs in stable, overactive, and normal detrusors were analyzed with RT-PCR. Results The ultramicrostructure of the ICCs changed significantly. The expression of c-kit was significantly higher in the overactive group than that in stable and normal groups (P