Localization and mRNA expression of dopamine receptor subtype D_4 and D_5 in human stomach and duodenum / 中华消化杂志

ABSTRACT

Objective To investigate the localization and mRNA expression of dopamine receptor subtype D_4 and D_5 mRNA in human stomach and duodenum by in situ hybridization. Methods The oligonucleotide probe was synthesized and labeled with digoxin, and then hybridized with human gastric and duodenal samples, which were stored in liquid nitrogen, fixed in hypothermia, and embedded in paraffin. The results were recorded and quantitatively analyzed using the pathological image computer analysis system. Results Dopamine receptor subtype D_4 and D_5 mRNA both expressed in human stomach as well as duodenum. They distributed mainly in the interstitial of mucosal glands and the lamina propria near lamina muscularis mucosa. The mRNA expression value of D_4 was higher than that of D_5 in both stomach and duodenum. In stomach, the number of positive target cells was 41.29?5.06 vs. 26.25?5.82 ( P