Determination of Total Alkaloids in Semen Strychni and Optimization of Its Extraction Conditions by Orthogonal Design / 中药新药与临床药理
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-571892
Responsible library:
WPRO
ABSTRACT
Objective To establish the RP- HPLC method for the determination of total alkaloids in Semen Strychni and to optimize its extraction conditions by orthogonal design. Methods With strychnine and brucine content as the indexes, ethonal volume, ethonal concentration , pH and extracting time were screened and L9(34) orthogonal test were applied. Results The optimal extraction conditions were as follows refluxing and extracting with 6- fold 50 % alcohol (pH=5) for 3 times, one hour for each time. Conclusion The optimized condition is the best for the extraction of alkaloids and this increase the availability of Semen Strychni.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Document type:
Article