Determination of tanshinone Ⅱ_A and cryptotanshinone in Compound Danshen Tablet by RP-HPLC / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-573338
Responsible library:
WPRO
ABSTRACT
AIM:
To set up a determination of tanshinone Ⅱ_A and cryptotanshinone in Compound Danshen Tablet(Radix Salviae Miltiorrhizae, Radix Notoginseng, Borneolum Syntheticum) by RP-HPLC.METHODS:
Two components could be separated through YWG-C_~18 column with methanol-water (75∶25 by volume) as a mobile phase. The flow rate was 1.0 mL?min~-1 , and the detection wavelength was at 270 nm.RESULTS:
The linear range of tanshinone Ⅱ_A and cryptotanshinone were 2.5-50.0 ?g?mL~-1 and 0.625-12.5 ?g?mL~-1 , respectively. The average recovery was 99.76% with RSD 0.92% for tanshinone Ⅱ_A and 98.34% with RSD 0.71% for cryptotanshinone, respectively.CONCLUSION:
The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of Compound Danshen Tablet.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Document type:
Article