Determination of Naringin and Neohesperidin in Fructus Aurantii by RP- HPLC / 中药新药与临床药理

ABSTRACT

Objective To establish a RP- HPLC method for the determination of naringin and neohesperidin in Fructus Aurantii. Methods The samples were extracted with methyl. The chromatographic conditions were as follows Hypersil C18 Column( 250 mm? 4.6mm, 5 ? m) , mobile phase of acetonitrile- water(pH=3.0, volume ratio of 19 ∶ 81), detection wavelength at 283 nm, flow rate being 1.0 mL/min and the column temperature at 25 ℃ . Results The calibration curves were linear in the range of 0.156～ 0.934? g for narngin (r=0.9990) and 0.155～ 0.930 ? g for neohesperidin (r=0.9991) . The average recovery of naringin was 101.62 % (RSD=1.91 % ) , and that of nephesoeridin was 103.12 % (RSD=1.22 % ) . Conclusion This method is simple, accurate and reliable for the quality control of Fructus Aurantii.