Effect of secretory leukocyte protease inhibitor on growth regulation of dermal mesenchymal stem cells / 重庆医科大学学报

ABSTRACT

Objective:To investigate the expression of SLPI in dMSCs and the effect of SLPI on growth regulaton of dMSCs.Method:Isolated stem cells from the rat skin tissue.Two pieces of sterile polyvinyl alcohol sponges were inserted subcutaneously in the dorsum of the rat and collected the wound fluid 1day after injury.Isolated RNA of two groupsdMSCs before and after the stimulation by wound fluid;skin tissue before and after trauma.SLPI was detected to know how they changed in dMSCs and in the skin before and after the injure by using RT-PCR and Western blot method.[H3] Thymidine incorporation was used to evaluate the mitogenic influence of SLPI.Results:Most of the neonatal dMSCs were spindle-shaped cells and exhibited multilineage potential in vitro.It was shown in the result of RT-PCR and western blot that SLPI expressed in dMSCs and increased after the cells being stimulated by wound fluid.A concentration-dependent proliferation promotion were demonstrated in dMSCs incubated for 24h in the prsense of SLPI.Conclusions:SLPI can be detected in dMSCs and support the growth of dMSCs in serum-free medium in vitro.In view of the multiple functions of SLPI,we supposed that it acted as an important role when dMSCs participated in the reparing of cutaneous injury.