Quantitative determination of paeoniflorin and puerarin in Rg soft-exact by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-576849
Responsible library:
WPRO
ABSTRACT
AIM:
This paper described a HPLC method.Paeoniflorin and puerarin in Rg soft-exact(Radix Puerariae lobatae,Redix Paeoniae alba,etc.) were simultaneously determined.METHODS:
A simplified HPLC procedure was developed.A 4.6 mm?250 mm Alltima C_(18) column with a mobile phase consisting of NaH_2PO_4(20 Mm,pH 2.4)-AcetonitrileTetrahydrofuran(9001205) was used.The flow rate was 1.00 mL/min.It was detected at 320 nm.RESULTS:
Paeoniflorin and puerarin were well separated.The average recoveries of paeoniflorin and puerarin were 98.10% and 101.2% respectively.CONCLUSION:
The result shows that this method is convenient,rapid and accurate.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Document type:
Article