Effect of inhibiting c-raf-1 on radiosensitivity in human cervical carcinoma cell line HeLa / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)2007.
Article
in Zh
| WPRIM
| ID: wpr-577130
Responsible library:
WPRO
ABSTRACT
Objective:To explore the effect of inhibiting c-raf-1 by antisense technology on radiosensitivity in human cervical carcinoma cell line HeLa.Methods:There were four groups in the study:control group,lipofectin group,sense oligodexynucleotides(S-ODN) group,antisense oligodexynucleotides(AS-ODN) group.The expression of c-raf-1 was detected by means of RT-PCR,Cellular response to irradiation was evaluated by the colony forming test,Apoptosis was observed by fluorescent staining.Bcl-2 protein expression was determined by flow cytometry.Results:AS-ODN group could significantly decreased the proliferation rate and increasing the apoptosis rate,significantly downregulating the expression of Bcl-2 of irradiated human cervical carcinoma cells,(vs lipofectin group or S-ODN group,P
Full text:
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Database:
WPRIM
Language:
Zh
Journal:
Journal of Chongqing Medical University
Year:
2007
Document type:
Article