Inhibition of antisense oligonucleotide targeting survivin on the proliferation of K562 cells / 重庆医科大学学报

ABSTRACT

Objective:To study the effect of survivin,an inhibitor of apoptosis,on the proliferation of leukemic cell by antisense oligonucleotide(ASON) technique.Methods:1.K562 cells were divided into 5 groups for cell inhibition rate experiment,111nmol/L ASON group,333nmol/L ASON group,555nmol/L ASON group,777nmol/L ASON group and the blank control group.At 24h,48h and 72h after transfection cells were collected to be stained by tapan-blue,and than the number of live cells and the rate of inhibition were counted.2.K562 cells were divided into 4 groups for the cell cycle and survivin protein expression,555nmol/L ASON group,544nmol/L NSON group,lipofectin group and the blank control group.At 24h,48h after transfection,the cell cycle progression was detected by flow cytometry,and the expression of survivin was examined by immunohistochemistry.Results:The rate of inhibition in ASON groups with different concentration was significantly higher than that of the control group,and the rate of inhibition was positively related to concentration of ASON and time of transfection.In 555nmol/L ASON group,the cell number in G0/G1 phase increased,and the cell cycle progression was inhibited significantly;while cell cycle progression in NSON group,lipofectin group and the control group had no significant change(P