Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell / 重庆医科大学学报

ABSTRACT

Objective:To explore the effect of liposomal transfection of survivin siRNA on the proliferation and apoptosis of lung adenocarcinoma cell lines A549.Method:Survivin siRNA was synthetized and transfected into A549 cell by liposome.Cell morphological change was observed with inverted fluorescent microscope,cell proliferation was examined by MTT assay at 24h 48h and 72h after transfection.cell proliferation and apoptotic index detected by flow cytometry and survivin mRNA expression determined by RT-PCR.Results:For the A549 cell transfected with siRNA cell proliferation was ihibited significantly,cell apoptosis appeared obvious and the expression of survivin mRNA significantly decreased.Conclusion:Survivin siRNA is able to inhibit the proliferation of lung adenocarcinoma cell lines A549 and induce the cell apoptosis.Survivin might become a new target for lung carcinoma gene therapy.