Chemistry fingerprints of water-soluble constituents of Salvia miltiorrhizae Bge.by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-577885
Responsible library:
WPRO
ABSTRACT
AIM:
To establish the HPLC-fingerprint of the water-soluble constituents of Salvia miltiorrhizae Bge.on 18 batches from 9 areas in China.METHODS:
Samples of Salvia miltiorrhizae Bge.from different areas were determined by Agilent 1100 DAD-HPLC on Sino Chrom ODS-BP column(250 mm?4.6 mm,5 ?m),gradient elution with methanol-water(0.7%H_3PO_4) as mobile phase,column temperature was at 30℃,flow rate was 1 mL/min,wavelength was at 280 nm,and the inject volume was 20 ?L.RESULTS:
The HPLC-fingerprints of the water-soluble constituents from Salvia miltiorrhizae Bge.,including 12 mutual peaks,were developed after determination of 10 batches of drugs according to SPSS analysis.,and in which,3 characteristic components were recognized.CONCLUSION:
The separation effect of peaks in fingerprints is better and the characteristic is also obvious.The RSD values of precision and reproducibility are less than 5%.This method can be used for the quality control of Salvia miltiorrhizae Bge.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Document type:
Article