Effects of troglitazone on cytokines induced expression of ICAM-1 in human mesangial cell / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effects of troglitazone on cell proliferation and expression of intercellular adhesion molecule-1(ICAM-1) induced by tumor necrosis factor-?(TNF-?) in human mesangial cells.Methods:Human mesangial cells were cultured in RPMI-1640 media containing TNF-? with or without troglitazone.Cell proliferation was assessed by MTT;the mRNA and protein expression of ICAM-1 were measured by RT-PCR and ELISA;the protein expression of NF-?B was measured by immunohistochemistry.Results:Troglitazone inhibited the proliferation of mesangial cell.The mRNA and protein expression of ICAM-1 of the cell induced by TNF-? increased significantly(P