Content Determination of 3-Epibetulinic Acid in Nodus Nelumbinis Rhizomatis Charcoal / 中国中医药信息杂志
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-579703
Responsible library:
WPRO
ABSTRACT
Objective To establish a method for the content determination of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal. Methods HPLC was used on Lichrospher C18 column (4.6 mm?150 mm, 5 ?m) with mobile phase of acetonitrile-0.2% glacial acetic acid (75∶25), the flow rate of 1 mL/min, the column temperature of 30 ℃ and the detection wavelength of 207 nm. Results 3-epibetulinic acid has a good linearity within 0.365 4~3.654 0 ?g (r=0.999 6) and the average recovery was 98.83% with RSD=2.47%. The content of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal from different region varied from 0.048 3% to 0.575 8%. Conclusion This method is simple, accurate, with good reproducibility, and can be used for the content determination of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Information on Traditional Chinese Medicine
Year:
2006
Document type:
Article