Variation in flow rates of anthraquinones in Anzhong Tongbian Capsule / 中成药

ABSTRACT

AIM: To establish variation in flow rates of anthraquinones in Anzhong Tongbian Capsule(Radix et Rhizoma rhei,Radix paeoniae alba,Fructus cannabis and Semen trichosanthis). METHODS: The thin layer chromatographic method was adopted. The anthraquinones compounds from Radix et Rhizoma Rhei in Anzhong Tongbian Capsules were extracted by ultrasound and separated by outspread on silica gel G thin layer,and n-hexane-ethyl acetate-formic acid(30 ∶ 10 ∶ 0. 5)as the developing system,detection wavelength was set at 435 nm and reference wavelength was 610 nm by using CAMAG TLC ScannerⅢ. RESULTS: Anthraquinones was separated well and 5 peaks of emodin,chrysophanol,aloe-emodin,rhein and physcion were ascertained. CONCLUSION: The method is simple and accurate with a good reproducibility and can be used for the quality control for Radix et Rhizoma Rhei in Anzhong Tongbian Capsules.