Efficient Generation of Human B7(CD80) Recombinant Adenovirus by COS/TPC Homologous Recombination / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6)1994.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-581782
Responsible library:
WPRO
ABSTRACT
The human CD80 full-length encoding cDNA was cloned by RT-PCR, and inserted into El-substituted aden-ovirus vector pAxlcw. Subsequently, the hCD80 recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I-digested Ad5-TPC, and the replication-deficient hCD80 recombinant adenovirus was generated efficiently by homologous recombination, with the tilers of 4?10~9pfu/ml. Infected with prepared hCD80 recombinant adenovirus in vitro, Hela cells expressed high levels of hCD80 48 hours after infection. These suggest that COS/TPC is an efficient method to prepare recombinant adenoviruses and the prepared hCD80 adenovirus could be used in cancer gene therapy.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1994
Document type:
Article