Rapid detection of Escherichiacoli with test paper of Na p-nitrophenyl ?-D-Glucuronate / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12)2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-582089
Responsible library:
WPRO
ABSTRACT
Objective To develop a new method for rapid detection of E.Coli.with a new substrate Na p nitrophenyl ? D glucuronate (PNP Glucu Na). Method The test paper was immersed with of Na p nitrophenyl ? D glucuronate which was synthesized by us and another paper with culture medium and phorphate buffer.Colour change was observed to detect the E.coli. Results An E.coli concerntration of 4?10 7 took 0.5 hour, while a concentration of 4?10 0 took 12 hours. 138 strains of E.coli and 190 strains of other bacteria were examined . The sensitivity was 94.2% and the specificity was 92.6%. Conclusion This method is rapid, accurate, simple, and economical for detecting E.coli.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Laboratory Medicine
Year:
2001
Document type:
Article