Serial Expression of Human ?,? globin Genes in Escherichia coli / 中国输血杂志
Chinese Journal of Blood Transfusion
; (12)1988.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-582102
Responsible library:
WPRO
ABSTRACT
Objective To make an attempt to express serialy the human ?,? globin in Escherichia Coli in order to carry out the research on blood substitute based on the genetic recombination of hemoglobin.Methods The routine molecular biology technology was used.Results The serial genetic fragment of ?,? globins containing the hemoglobin was amplified by PCR.The genetic sequencing showed that there was no unexpected mutation.And then,the serial genes were cloned into the pBV220 expression carrier.Undergoing thermal inducement,the expression product could reach about 20% of total bacterial protein.The expression product assumed the form of inclusion body,and it was vertified by Western Blotting.Conclusion This much can lay the solid foundations for further launching the research on expression and pruification of recombinant human hemoglobin genes.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Blood Transfusion
Year:
1988
Document type:
Article