Comparative Effect of Different Media in in vitro Cultivation of Trichomonas vaginalis / 中国寄生虫学与寄生虫病杂志

ABSTRACT

Objective To investigate the optimal condition for in vitro cultivation of Trichomonas vaginalis for obtaining a better harvest of T^vaginalis. Methods An isolate of T^vaginalis from clinical specimens was cultivated in three different media with initial inoculation of 9^0?10\+4/ml under pH 5^6. Results There was distinct difference after 96h incubation in the cumulative harvest of T.vaginalis. The highest harvest was received in cysteine/liver/peptone/maltose medium, followed by the liver/peptone/maltose medium and soybean/liver/peptone/maltose medium. Conclusion The cysteine/liver/peptone/maltose medium may be a suitable environment for in vitro multiplication of T.vaginalis.