Improved detection of Mycobacterium leprae rifampin resistance with nested-PCR / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12)2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-585330
Responsible library:
WPRO
ABSTRACT
Objective To improve the sensitivity of PCR for studying the rifampin resistant gene (rpoB) of M.Leprae from clinical samples.Method After comparing the results of PCR with Q-Solution (PCR Enhancer) and without Q-solution,Nested-PCR was then applied for the detection of rpoB gene.Results Although PCR Enhancer improves the amplification of rpoB gene, the sensitivity of routine PCR is only 45. 2%.The sensitivity of Nested-PCR for detecting mutants in rpoB gene can further increase to 90.5% after selection of optimum parameters.Conclusion The combinaion of PCR Enhancer and Nested-PCR improves the sensitivity and specificity of PCR for detection of rifampin resistant gene of M. Leprae.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Leprosy
/
Neglected Diseases
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Laboratory Medicine
Year:
2003
Document type:
Article