Composition and cytotoxic activity of the organic acids from Gracilaria lemaneiformis / 中国海洋药物

ABSTRACT

Objective To investigate the composition and cytotoxic activity of the organic acids extracted from a red alga Gracilaria lemaneiformis.Methods Growth inhibition rate of the extract on HeLa cell was determined by MTT assay.The composition and contents of the organic acids were analysed by GC-MS after methylied.Results The organic acids from Gracilaria lemaneiformis showed potent cytotoxic activity against HeLa cell,IC_(50)=6.5?g? mL~(-1).Thirteen compounds were characterized from methylied organic acids.The major constituent was palmitic acid methyl ester (61.34%).Their relative acids were mainly C_(16)or C_(18)fatty acids and phthalates.Of them,palmitic acid,stearic acid,palmitoleic acid,oleic acid and linoleic acid have been reported to show antitumor activity.Conclusion The fatty acids mentioned above maybe a part of antitumor components of Gracilaria lemaneiformis.