Effect of rhG-CSF mobilized peripheral blood and bone marrow after being mixed in different proportions on immunologic properties of grafts / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-586466
Responsible library:
WPRO
ABSTRACT
Objective To explore the immunologic composition and T cell function of rhG-CSF mobilized peripheral blood grafts(G-PB) and rhG-CSF primed bone marrow grafts(G-BM) after being mixed in different proportions.Methods T cell subgroups,dendritic cells(DC) and its subsets in G-PB and G-BM were analyzed by multicolor flow cytometry.Immunologic composition of G-PB and G-BM after mixture in different proportions were calculated,lymphocyte proliferation ability and quantities of interleukin-4(IL-4),interferon-?(IFN-?) secretion by T cells in mixture grafts were determined using MTT and sandwich ELISA assays.Results After co-incubation of G-PB with G-BM in different proportions,T cell proliferation in mixture grafts was lower than that of NG-BM and G-PB(P
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Basic & Clinical Medicine
Year:
2006
Document type:
Article