Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
; : 99-107, 2006.
Article
in En
| WPRIM
| ID: wpr-58676
Responsible library:
WPRO
ABSTRACT
PURPOSE: Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer. MATERIALS AND METHODS: Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3~10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D software(TM). The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases. RESULTS: A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins weredown-regulated. Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, alpha-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study. CONCLUSION: 2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Apolipoproteins
/
Phosphopyruvate Hydratase
/
Carcinoma, Squamous Cell
/
Biomarkers
/
Uterine Cervical Neoplasms
/
Cervix Uteri
/
Mass Screening
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Annexin A2
/
Databases, Protein
/
Electrophoresis, Polyacrylamide Gel
Type of study:
Screening_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Cancer Research and Treatment
Year:
2006
Document type:
Article