Potential roles of the C-ERBB_2 protooncogene in mouse oocyte maturation and in conducting EGF promoting mouse oocyte maturation in vitro / 基础医学与临床

ABSTRACT

Objective Potential roles of the C-ERBB_(2) protooncogene in mouse oocyte maturation and in conducting Epidermal Growth Factor(EGF) promoteing mouse oocyte maturation in vitro were investigated.Methods This research used mouse oocyte culture model and RT-PCR method to study the effects of both(C-ERBB_(2) and EGF(on mouse) oocyte maturation in vitro,and the potential role of the C-ERBB_(2)protooncogene on EGF effect.Results(C-ERBB_(2) ASODN inhibited germinal vesical breakdown(GVBD) and the first polar(PBⅠ) extrusion of denuded(oocytes(DOs) in a dose-dependent and time-dependent way,and delayed their maturation significantly.C-ERBB_(2) ASODN also inhibited the effect of EGF on oocyte maturation in a time-dependent way.In GVBD oocytes,RT-PCR showed that C-ERBB_(2) mRNA was expressed in oocytes and the level of (C-ERBB_(2) mRNA) was the highest in(EGF treated) group,midst in EGF+c-erbB_2ASODN treated group,and lowest in C-ERBB_(2) ASODN treated group.Conclusion C-ERBB_(2) exists in the oocyte and has promoting effect on oocyte maturation in vitro.EGF plays a rolein inducing denuded oocyte maturation through up-regulating C-ERBB_(2)expression of oocyte.Interfering C-ERBB_(2) expression can reduce the effect of EGF on oocyte maturation.