Changes and their relationship of aquaporin-9 mRNA expression and Ca~(2+) concentration after cerebral ischemic in rats / 临床神经病学杂志

ABSTRACT

Objective To investigate the changes and their relationship of aquaporin-9(AQP9) mRNA expression and Ca2+ concentration of brain tissue after cerebral ischemic.Methods The models of cerebral ischemic in the rats were made by occluding unilateral middle cerebral artery with the suture method.The expression level of AQP9 mRNA was assessed by RT-PCR at interval times of 6 h,1 d,2 d,3 d,5 d after cerebral ischemic,respectively.Fura-2/AM fluoremetry technique was used to determine the cellular Ca2+ concentration of brain tissue.The results were compared with control group.Results Compared with control group,the expression level of AQP9 mRNA and the concentrations of Ca2+ significantly increased at 6 h in ischemic edema tissue,and reached a peak at 2 d,3 d after cerebral ischemic(P