Influence of vascular endothelial growth factor versus basic fibroblast growth factor on radial fracture healing in rabbits / 中国组织工程研究

ABSTRACT

BACKGROUND: Studies have demonstrated that vascular endothelial cell growth factor (VEGF) and basic fibroblast growth factor (bFGF) play important roles in entochondrostosis and fracture healing blood vessel hyoperplasia. OBJECTIVE: To observe and compare the roles of VEGF and bFGF in fracture healing. DESIGN, TIME AND SETTING: Double-factor trial was performed at Pathological Laboratory, North China Coal Medical University from August 2005 to May 2006. MATERIALS Twenty-four adult healthy Japan rabbits were selected and radial fracture models were created in 48 bilateral anterior limbs. The rabbits were randomly divided into VEGF group and bFGF group (n=24). METHODS: VEGF (0.2 ?g) and bFGF (100 ng) were injected respectively in the fracture site of rabbits. No external fixation was used. MAIN OUTCOME MEASURES: The rabbits were executed at postoperative weeks 2, 4 and 6 to measure callus sagittal diameter, transverse diameter and section area. X-ray was used to observe fracture healing and measure bony callus total area. Histological alterations during fracture healing were observed, and percentage of trabecular bone, cartilage and fibrous tissue in the bony callus was determined. RESULTS: At 2 weeks after operation, callus sagittal diameter and transverse diameter in VEGF group were significantly larger than bFGF group (P