Reference genes selection in mRNA analysis of aging rat tissues / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-593669
Responsible library:
WPRO
ABSTRACT
Objective To investigate the proper inner control genes suitable for mRNA expression level comparison of aging rat tissues.Methods Real-time reverse transcription PCR was used to examine in aging rat tissues the expression level of G3pd(glyceraldehyde-3-phosphate dehydrogenase),ACTB(?-actin),H3f3b(H3 histone,family 3B),Arbp(acidic ribosomal phosphoprotein P0)and 18S(18S ribosomal RNA).Results The most stably expressed housekeeping gene in aging rat kidney was ACTB,in heart and lung G3pd showed the minimum variation;Arbp expression was the most stable one in different tissues.Conclusion For aging rat intra-tissue mRNA normalization at least two housekeeping genes should be used one is the ribosomal RNA gene 18S and another one is Arbp.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2006
Document type:
Article