Effects of recombinan human granulocyte colony-stimulating factor on brain edema and vascular regeneration in rats after intracerebral hemorrhage / 中国组织工程研究

ABSTRACT

BACKGROUND:Administration of recombinant human granulocyte colony-stimulating factor(rhG-CSF) is known to diminish cerebral edema and to enhance the new vascularization by mobilizing endothelial progenitor cells in cerebral infarction, then to promote the neurofunctional recovery.OBJECTIVE:To investigate the effects of rhG-CSF on brain edema and new vessels after intracerebral hemorrhage(ICH) in rats.DESIGN, TIME AND SETTING:The randomized controlled animal study was performed at the Central Laboratory, Luzhou Medical College from March to November 2006.MATERIALSA total of 144 healthy male Sprague Dawley rats were equally and randomly assigned into a sham operation group, a ICH group and a treatment group.rhG-CSF(Xinpeng, Shenzhen, China) was used in this study.METHODS:Rat models of ICH were made by the method of cutting off the tail of each rat to obtain autoblood in the ICH and treatment groups.Rats in the sham operation group were injected with saline.Rats in the treatment group were administered with rhG-CSF(60 ?g/kg) by intraperitoneal injection after 1 hour.Eight rats from each group were studied at 6, 12, 24, 48, 72 hours and 7 days.Brain water content of rats was measured by dry-wet method.CD34+ vessel expression was detected by SP and DAB coloration, immunohistochemical method.MAIN OUTCOME MEASURES:Dynamic changes in brain water content;immunohistochemical results of CD34+ vessels.RESULTS:The water contents were significantly higher in the ICH group than in the sham operation group(t=4.49, P