A new model-tracking of ?-cell development by generation of transgenic zebrafish / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-595285
Responsible library:
WPRO
ABSTRACT
Objective To establish a pancreatic ?-cell developmet fish model with specific spatial expression patterns.Methods Molecular cloning,microinjection,whole embryo in-situ hybridization(WISH)and fluorescence microscopy in living were used to analyze of ?-cell development through generation of transgenic zebrafish.ResultsScreened and established pancreatic ? cells of transgenic zebrafish,and confirmed the fluorescence protein expression in the same spatiotemporal pattern with endogenous insulin gene to achieve dynamic monitoring islet ?-cell development situation in vivo.Conclusion The pancreatic ? cells of transgenic zebrafish animal model can successfully trace pancreatic ? cell development.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Basic & Clinical Medicine
Year:
2006
Document type:
Article