The complete reversal of mulitidrug resistance in leukemia cells by shRNA / 中国基层医药

ABSTRACT

Objective To study the efficiency of reversing MDR by suppressing MDR1 gene with RNAi in K562/ADM cells. Methods For reversing MDR by RNAi technology, two different short hairpin RNAs (shRNAs) were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into the highly adriamycin-resistant K562/ADM cell line. The RNAi effect on MDR was evaluated by real-time PCR, and Rhodamine123 (Rh123) efflux assy. Results The stable transfected clones showed varied degrees of reversal of MDR phenotype. Surprisingly, the MDR phenotype was completely reversed in two transfected clones. Conclusion This study demonstrates that MDR can be reversed by the shRNA-mediated RNAi in K562/ADM cells, which provides a valuable clue as to sensitizing multidrug-resistant hepatoma cells to anti-cancer drugs.