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Simultaneous Determination of Ferulic Acid and Paeoniflorin in Ankong Zhongzi Wan by Dual Wavelength HPLC / 中国中医药信息杂志
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-598462
Responsible library: WPRO
ABSTRACT
Objective To determine ferulic acid and paeoniflorin in Ankong Zhongzi Wan by HPLC under dual wavelength ultraviolet detection. Methods Ferulic acid and paeoniflorin were separated by Waters SymmetryShield-C18 column (4.6 mm × 250 mm, 5 μm) with gradient elution of acetonitrile-0.1%phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 230 nm and 323 nm. Results The linear relationship of ferulic acid and paeoniflorin was good in the range of 0.058 2-0.582 4 μg (r=0.999 4) and 1.664-16.64 μg (r=0.999 6), and the average recovery rate was 97.77% (RSD=1.88%) and 98.84% (RSD=1.96%), respectively. Conclusion The method is accurate and quick for determining the two effective components in Ankong Zhongzi Wan, and can be used for its quality control.

Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Information on Traditional Chinese Medicine Year: 2013 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Information on Traditional Chinese Medicine Year: 2013 Document type: Article
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