Determination of 23-Acetate Alisol B in Longdan Xiegan Honey Pills by HPLC / 中国药师
China Pharmacist
; (12): 2149-2150,2151, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-600064
Responsible library:
WPRO
ABSTRACT
Objective:
To establish a method for the determination of 23-acetate alisol B in Longdan Xiegan honey pills by HPLC.Methods:
The analysis was performed on a Waters Symmetry C18 (250 mm × 4. 6 mm,5μm) column with the mobile phase of acetonitrile-0. 1% phosphoric acid (62 ∶ 38). The flow rate was 1. 0 ml·min-1, the column temperature was 35℃ and the detection wavelength was set at 208nm.Results:
The linear range of 23-acetyl alisol B was 19. 999 5- 1 999. 9500 ng(r =0. 999 9), and the average recovery was 95. 56%(RSD = 0. 7%, n = 6).Conclusion:
The method is simple, rapid and accurate, and can be used to control the quality of Longdan Xiegan honey pills with good repeatability and recovery.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Document type:
Article