Experimental study on determination of viability of Schistosoma japonicum cercariae by staining / 中国血吸虫病防治杂志
Chinese Journal of Schistosomiasis Control
; (6): 64-65,69, 2015.
Article
in Zh
| WPRIM
| ID: wpr-600487
Responsible library:
WPRO
ABSTRACT
Objective To determine the viability of Schistosoma japonicum cercariae by staining. Methods Schistosoma japonicum cercariae were stained by 0.4%trypan blue 0.5%methylene blue?eosin?borax M.E.B 0.5%eosin 0.5%methy?lene blue and 0.05% neutral red respectively for 5 min then they were observed under a stereoscopic microscope. Results The dead cercariae were stained in the trypan blue M.E.B eosin and neutral red but unstained in the methylene blue. The vi?tal cercariae were unstained in all the five kinds of dyes. Conclusion The staining methods by using 0.4% trypan blue 0.5%M.E.B 0.5%eosin and 0.05%neutral red can be used to determine the viability of S. japonicum cercariae.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Schistosomiasis Control
Year:
2015
Document type:
Article