Application of flow cytometric score in the diagnosis of myelodysplastic syndromes / 白血病·淋巴瘤
Journal of Leukemia & Lymphoma
; (12): 530-533,537, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-600511
Responsible library:
WPRO
ABSTRACT
Objective To identify myelodysplastic syndromes (MDS) from non-MDS (mainly non-clonal cytopenias) by flow cytometric (FCM) score.Methods Four parameters (CD34+ myeloblast-related and B-progenitor-related cluster size,myeloblast CD45 expression and granulocyte side scatter value) were rated on 50 patients with MDS and 20 patients with non-clonal cytopenias.A diagnosis of MDS was formulated in the presence of FCM score value ≥ 2 (every abnormal parameter was scored 1).Results When compared with non-clonal cytopenias,patients with MDS had increased myeloblast-related cluster size and decreased B-progenitor-related cluster size,and had a lower granulocyte side scatter (P < 0.05),but no significant difference was found when myeloblast CD45 expression was compared (P =0.06).The FCM score value was significant higher in MDS patients (P < 0.001).45 cases were correctly classified (sensitivity 90 %).There was 1 false-positive case among 20 controls (specificity 95 %).Conclusion The FCM score may help to establish the diagnosis of MDS,and it is simple and feasible.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Journal of Leukemia & Lymphoma
Year:
2014
Document type:
Article