Role of NF-κB siRNA in MCP-1 levels induced by IL-17 stimulation in cardiac myocytes / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 1333-1336,1346, 2015.
Article
in Zh
| WPRIM
| ID: wpr-602483
Responsible library:
WPRO
ABSTRACT
Objective:To investigate the effect of NF-κB p65siRNA of IL-17 inducing the expression of MCP-1 in the primary cultured cardiac myocytes.Methods:The cardiac myocytes were isolated from neonatal mice by different adhesion method.NF-κB P65 siRNA was transfected into cardiac myocytes and the rates of transcription and translation of MCP-1 were detected by RT-PCR and enzyme-linked immunosorbent assay( ELISA) ,the rates of transcription and translation of P-P65 and P65 were detected by RT-PCR and Western blot.Results:Compared with negative siRNA group,the expression of the MCP-1 at mRNA and protein levels were increased in negative siRNA +IL-17 group(P0.05 ) .Conclusion: IL-17 stimulates MCP-1 expression in cardiac myocytes via NF-κB activation,and NF-κB P65 siRNA can effectively inhibit the upregulation of IL-17 on MCP-1.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
2015
Document type:
Article