Investigation of hsa-miR95-5p,hsa-miR-548am-5p and hsa-miR-1246 in pathogenesis of gestational diabetes mellitus / 实用医学杂志

ABSTRACT

Objective To investigate the expression of microRNA (miRNA) in placenta tissue, explore the function of miRNA in pathogenesis of gestational diabetes mellitus (GDM). Methods Placenta tissue from pregnant women with GDM and normal controls were selected from January 2013 to January 2014. Expression of miRNAs were detected by sequencing technique and quantity real time PCR(qRT-PCR). Target genes of miRNA were analyzed by KEGG. Results 52 insulin signaling pathway related miRNAs including 47 up-regulated and 5 down-regulated were differentially expressed in GDM compared with normal control. Expression of placental hsa-miR-548am-5p and hsa-miR-95-5p were significantly increased , but hsa-miR-1246 was significantly lower (P <0.05) in GDM group than normal control; all the results were consistent with sequencing results. Key points of insulin signaling pathway could regulate expression of targets such as PI3K. Conclusion miRNA expression in GDM placenta was abnormal.A variety of exp ressions of miRNAs were involved in the pathogenesis of GDM by regulating insulin signaling pathway.