Simultaneous Determination of 3 Optical Isomers in Carfilzomib by HPLC / 中国药师
China Pharmacist
; (12): 1094-1095,1101, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-604346
Responsible library:
WPRO
ABSTRACT
Objective:
To establish an HPLC method for the determination of optical isomers in carfilzomib .Methods:
The sample was separated on a Chiralpak OX-H column.The mobile phase consisted of n-hexane∶isopropanol∶alcohol (89 ∶5 ∶6, v/v/v) with a flow rat of 1.0 ml· min-1 .The detection wavelength was 220 nm.Results:
The linear rang of enantiomer , diastereomer Ⅰand di-astereomer Ⅱwas 0.54-2.14 μg· ml -1,0.11-1.80 μg· ml-1 and 0.11-1.81 μg· ml-1(r≥0.998), respectively.The lower limit of quantification was 0.07-0.27 μg· ml-1 .The recoveries of optical isomers were within the range of 99.6%-100.9% with RSD of 1.13%-1.59%(n=9).Conclusion:
The method is sensitive, simple, fast, accurate and specific, and suitable for the study of opti-cal isomers in carfilzomib .
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Document type:
Article