Luteolin protects brain injury and improves endogenous neural stem cells proliferation on cerebral ischemia-reperfusion injury in rat / 中国生化药物杂志

ABSTRACT

Objective To investigate the protective effects of luteolin on cerebral ischemia-reperfusion injury in rats.Methods 40 SD rats were randomly divided into four groups:sham group,model group,luteolin low and high dose groups.Each group was given oral administration for 7 days.After 1 h of last administration,the CIRI ( cerebral ischemia-reperfusion injury) group was produced inserting 5-0 line into the internal carotid artery.Then levels of IL-10, IL-1βand TNF-αcontents in myocardial tissues were measured.Meanwhile,infarct size was detected by TTC staining and number of BrdU +and Nestin + were measured by immunohistochemical methods.Results Compared with model group, luteolin could significantly decrease the levels of TNF-αand IL-1βin cerebral tissues(P<0.05),improve IL-10 content in cerebral tissues(P<0.05),and effectively reduce myocardial infarct range(P<0.05).Meanwhile luteolin could improve the expressions of BrdU + and Nestin + in cerebral tissues(P <0.05).Conclusion Luteolin has beneficial effects on rats with cerebral ischemia-reperfusion injury and improves endogenous neural stem cells proliferation.