Temporal and subcellular distributions of Cy5.5-labeled hyaluronic acid nanoparticles in mouse organs during 28 days as a drug carrier
Korean Journal of Veterinary Research
; : 215-222, 2017.
Article
in En
| WPRIM
| ID: wpr-60682
Responsible library:
WPRO
ABSTRACT
Temporal and subcellular distributions of hyaluronic acid (HA) as a degradable nanoparticle (NP) in animals were investigated to determine if HA-NP could be utilized as an appropriate drug delivery system. After mice were intravenously injected with 5 mg/kg of Cy5.5-labeled HA-NP sized 350–400 nm or larger HA-polymers, the fluorescence intensity was measured in all homogenized organs from 0.5 h to 28 days. HA-NP was greatly detected in spleen, liver and kidney until day 28, while it was maintained at low levels in other organs. HA-polymer was observed at low levels in all organs. HA-NP quantities in spleen and liver were reduced until day 3, but increased sharply between days 3 and 7, then decreased again, while their HA-polymers were maintained at low levels until day 28. In kidneys, both HA-NP and HA-polymer showed high levels after 0.5 h of administration, but steadily decreased until day 28. According to ultrastructural analyses, HA-NP was engulfed in Kupffer cells of liver and macrophages of spleen and kidney at day 1 and was accumulated in the cytoplasm of kidney tubular cells at day 7. Overall, these findings suggest that HA-NP could be considered a desirable drug carrier in the liver, kidney, or spleen.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Spleen
/
Drug Carriers
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Pharmacokinetics
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Drug Delivery Systems
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Cytoplasm
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Nanoparticles
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Fluorescence
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Hyaluronic Acid
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Kidney
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Kupffer Cells
Limits:
Animals
Language:
En
Journal:
Korean Journal of Veterinary Research
Year:
2017
Document type:
Article