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Effect of culture supernatant of rat bone mesenchymal stem cells on biological characteristics of RSC96 cells / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 1291-1295, 2017.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-615115
Responsible library: WPRO
ABSTRACT

Objective:

To study the effect of culture supernatant of rat bone marrow mesenchymal stem cells (BMSCs) on biological characteristics of RSC96 Schwann cells.

Methods:

BMSCs of Sprague Dawley rat were cultured and purified in vitro.The BMSCs culture supernatant medium (BMSC-CM) was collected from the third generation of BMSCs.Using MTT method to detect proliferation influence of BMSC-CM on RSC96 cells,and using plate cloning assay to analysis a single cell proliferation effect.The migration analysis of BMSC-CM on RSC96 cells were detected by scratch and TranswellTM chamber assay,Western blot tested Bax and Bcl-2 proteins changes in RSC96 cells.

Results:

The third generation of BMSCs was long spindle-shaped and vortex-like;the result of Osteogenesis staining was positive;lipid droplets appeared after adipogenesis induction.BMSC-CM inhibited the proliferation and migration of RSC96 cells.Meanwhile,Bcl-2 protein decreased and Bax protein increased in RSC96 cells after BMSC-CM treatment.

Conclusion:

BMSC-CM can promote apoptosis and inhibit the proliferation and migration of RSC96 cells.

Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Immunology Year: 2017 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Immunology Year: 2017 Document type: Article
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