Laboratory diagnosis of the first imported case of Zika virus infection from Suriname into Guangdong,China / 中国人兽共患病学报
Chinese Journal of Zoonoses
; (12): 522-525, 2017.
Article
in Zh
| WPRIM
| ID: wpr-618025
Responsible library:
WPRO
ABSTRACT
We detected Zika virus (ZIKV) in a febrile case returning from Suriname and entry China from Guangzhou Baiyun International Airport Port.Serum and saliva samples were collected from a suspected case returning from Suriname.We detected ZIKV RNA using real-time fluorescence RT-PCR methods by both in-house reagent and commercial detection kits.RT-PCR detection was carried out with saliva sample and sequence analysis was performed.Phylogenetic tree was constructed to analyze the source of imported cases.Real-time fluorescent RT-PCR result showed that saliva was detected ZIKV RNA positive while for serum was weakly positive.A specific 1 500 bp fragment in size was amplified with saliva sample by RT-PCR.Sequence analysis showed 99% homologous to the corresponding sequence of Brazil ZIKV (GenBank No.KX197250).Phylogenetic tree indicated it was located on African lineage.According to the epidemiological investigation results,clinical manifestations and nucleic acid detection of case,the suspected case was confirmed to infect Zika virus,being the first case from Suriname into Guangdong Province.
Full text:
1
Database:
WPRIM
Type of study:
Diagnostic_studies
Country/Region as subject:
America do sul
/
Caribe ingles
/
Suriname
Language:
Zh
Journal:
Chinese Journal of Zoonoses
Year:
2017
Document type:
Article