Application value of combined detection of virus nucleic acid and antibody in HFMD / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 1488-1489,1492, 2017.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-619179
Responsible library:
WPRO
ABSTRACT
Objective To investigate the application value of combined detection of enterovirus 71 type(EV71) nucleic acid and antibody in diagnosis of hand-foot-mouth disease(HFMD).Methods From Jan.to Nov.2015,a total of 180 children with suspected HFMD were divided into nucleic acid detection group,antibody detection group and combined detection group,with 36 subjects for each group.Viral nucleic acid was detected by using reverse transcription polymerase chain reaction.Antibody was detected by using enzyme linked immunosorbent assay.All detected results were analyzed.Results The positive rate of nucleic acid detection group was the highest(80.55%),while that of antibody detection group was the lowest(47.22%).Among various symptoms,the positive rates of nucleic acid,antibody and combined detection in subjects with skin rash were the highest.Conclusion Combined detection of EV71 nucleic acid and antibody could avoid the false positive result of nucleic acid detection and false negative result of antibody detection,thus could be helpful for increasing diagnostic accuracy rate of HFMD.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
International Journal of Laboratory Medicine
Year:
2017
Document type:
Article