Optimization of Extractive Microbial Transformation for Producing L-phenylacetylcarbinol by Response Surface Method / 中国药房

ABSTRACT

OBJECTIVE:To optimize the conditions of extractive microbial transformation for producing L-phenylacetylcarbi-nol(L-PAC). METHODS:HPLC was used to determine the concentration of L-PAC,which was extracted by saccharmyces cerevi-siae. Using L-PAC concentration as response value,Box-Behnken response surface design was adopted to investigate the 3 main fac-tors of benzaldehyde,Triton X-100 and glucose dose,and verification test was conducted. RESULTS:The interaction between benzaldehyde and Triton X-100 was the most significant. The optimized combination was as follows as benzaldehyde of 1.1%,Tri-ton X-100 of 0.14 g/mL,glucose of 0.028 g/mL. The average concentration in verification test of L-PAC was 28.04 mmol/L (RSD=1.35%,n=3),showing 0.11% relative error with the predicted value of 28.01 mmol/L. CONCLUSIONS:Response sur-face method has optimized the conditions of extractive microbial transformation for producing L-PAC,obtained optimal combina-tion of each factor,and provided favorable reference for volume-producing L-PAC.