High glucose promotes the CTGF expression in human mesangial cells via serum and glucocorticoid-induced kinase 1 pathway / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 508-12, 2008.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-634937
Responsible library:
WPRO
ABSTRACT
The role of serum and glucocorticoid-induced kinase 1 (SGK1) pathway in the connective tissue growth factor (CTGF) expression was investigated in cultured human mesangial cells (HMCs) under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with pIRES2-EGFP-S422D hSGK1 (SD) could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with pIRES2-EGFP (FP) under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with pIRES2-EGFP-K127N hSGK1 (KN) could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Signal Transduction
/
Cells, Cultured
/
Protein Serine-Threonine Kinases
/
Immediate-Early Proteins
/
Mesangial Cells
/
Connective Tissue Growth Factor
/
Glucose
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2008
Document type:
Article