Mechanism of bradykinin on inflammations of retinal pigment epithelium cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
; (12): 1430-1434, 2016.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-637872
Responsible library:
WPRO
ABSTRACT
Abstract?AIM:
To investigate mechanism of bradykinin ( BK) on inflammations of retinal pigment epithelium ( RPE) cells.?METHODS:
ARPE -19 cells were cultured in vitro, stimulated by 100nM BK for 24h. Cell morphology changes were observed by microscope, and BK receptor localization was detected through cell immunofluorescence. Changes of Ca2+in BK and BR antagonist stimuli were detected by laser scanning confocal microscopy.The expressions of COX-1, COX-2, eNOS and iNOS protein in control group and BK group were detected by Western Blot.?RESULTS:
After the stimulation of BK, there was no significant changes of ARPE-19 cells in morphology.Kinin B1 receptors ( B1R ) and B2 receptors ( B2R ) could be detected in ARPE-19 cells.Compared with control group, Ca2+concentrations significantly increased in BK group; in B1R antagonist group and B2R antagonist group Ca2+concentrations increased less than BK group; B1R and B2R antagonist group showed no obvious changes in Ca2+concentrations.Compared with control group, COX-2 and iNOS protein concentrations were significantly increased in BK group (P<0.001).?CONCLUSION:
BK induces the increasing expression of COX-2 and iNOS in the cultured ARPE cells through binding with either B1R or B2R.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2016
Document type:
Article