Changes of brain - derived neurotrophic factor expression in gene modified bone marrow mesenchymal stem cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
; (12): 1816-1819, 2016.
Article
in Zh
| WPRIM
| ID: wpr-637949
Responsible library:
WPRO
ABSTRACT
AIM: To study the changes of brain - derived neurotrophic factor ( BDNF ) expression in gene modified bone marrow mesenchymal stem cells ( BMSC) . ●METHODS:BMSC were divided into blank control group ( without transfected BMSC ) , negative control group ( empty vector without BDNF gene transfected BMSC) and experimental group ( BDNF gene transfected BMSC) . The expression of BDNF mRNA in BMSC was measured by Realtime PCR, and the expression of BDNF in BMSC was measured by ELlSA. ●RESULTS:The BDNF mRNA expressions of 3, 4, 5, 6, 7 and 8-generation BMSC cells in the experimental group were higher than those in the blank control group and negative control group. The differences were statistically significant (P3: F=491. 788, P ●CONCLUSION:Long-term expression of BDNF in BMSC can be enhanced by genetic engineering.
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Database:
WPRIM
Language:
Zh
Journal:
International Eye Science
Year:
2016
Document type:
Article