Expressions of Interferon Gamma and Transforming Growth Factor Bata1 in Hyperoxia-Induced Lung Injury and Their Significances / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-638807
Responsible library:
WPRO
ABSTRACT
Objective To observe the dynamic changes of interferon gamma(IFN-?) and transforming growth factor bata1(TGF-?_1) in animal model of hyperoxia-induced lung injury,and to explore the mechanism of fibrosis.Methods Thirty-two juvenile Wistar rats were randomly divided into room-air group and hyperoxia group,the hyperoxia group were further divided into 3 subgroups of hyperoxia-exposure for 3,7 and 14 days.The mRNA levels of IFN-? and TGF-?_1 in the lung were measured by reverse-transcription polymerase chain reaction(RT-PCR).The expressions of IFN-? and TGF-?_1 protein were measured by immunohistochemical(stai)-ning.Results The level of IFN-? mRNA of groups under hyperxia-exposure for 3,7 and 14 days were higher than that of control group.The expression of IFN-? mRNA notably elevated and was up to its peak(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Applied Clinical Pediatrics
Year:
2004
Document type:
Article