The Gene Expression of Dynorphin, Enkephalin, c-Fos in the Neuropathic Rat / 대한마취과학회지
Korean Journal of Anesthesiology
; : 293-303, 1996.
Article
in Ko
| WPRIM
| ID: wpr-63923
Responsible library:
WPRO
ABSTRACT
BACKGROUND: We studied the time course of gene expression of dynorphin, enkephalin, c-fos, and the changes of allodynia, and the effect of chemical sympathectomy on the gene expression and allodynia in neuropathic rat. METHODS: In two groups of rat (Sprague-Dawley), the left L5 and L6 spinal nerves were tight ligated. In gene expression group (N=25), behavioral tests for mechanical allodynia and cold allodynia were perfomed for the next two weeks. After the test of allodynia, the expression of dynorphin, enkephalin, c-fos were assessed by Northern blot hybridization. In chemical sympathectomy group (N=16), after chemical sympathectomy (guanethidine 70 mg/kg intraperitoneally, from postoperative 7 days to 9 days), the changes of allodynia and the gene expression of enkephalin, c-fos were tested. RESULTS: Mechanical allodynia and cold allodynia was developed on the postoperative 3, 5, 7, 14 days. Preprodynorphin mRNA expression was reached peak level at the postoperative 8 hrs, sustained increase by the postoperative 3 days, but preproenkephalin mRNA expression increased slightly after operation. c-Fos mRNA expression was increased immediately at the postoperative 30 min, 1 hr, returned to normal level thereafter, and increased again on the postoperative 3, 5, 7 days that neuropathic pain was developed. Mechanical allodynia and cold allodynia were decreased by chemical sympathectomy. The increased c-fos mRNA expression and pain at postoperative 7 days was reduced by chemical sympathectomy. CONCLUSION: These results suggest that the transient gene expression of dynorphin and c-fos after tight ligation of L5 and L6 spinal nerves induces the development neuropathic pain, and late c-fos expression is related to neuropathic pain.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Spinal Nerves
/
Sympathectomy, Chemical
/
RNA, Messenger
/
Dynorphins
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Enkephalins
/
Gene Expression
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Blotting, Northern
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Hyperalgesia
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Ligation
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Neuralgia
Limits:
Animals
Language:
Ko
Journal:
Korean Journal of Anesthesiology
Year:
1996
Document type:
Article