Expression and Significance of Matrix Metalloproteinase and Tissue Inhibitor of Metalloproteinase-1 in Rats with Glomerular Sclerosis / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-639456
Responsible library:
WPRO
ABSTRACT
Objective To investigate expression and significance of matrix metalloproteinase-2(MMP-2),MMP-9 and tissue inhibitor of metalloproteinase-1(TIMP-1) in rats with glomerular sclerosis made by doxorubicin.Methods Forty Wistar male rats(8-week-old) were randomly assigned into 2 groupssham operated and model groups.Rats in model group were nephrectomized after anesthesia and injected with adriamycin(5 mg/kg) after 1 week.Rats in sham operated group was subjected to sham operation and injected with normal saline after 1 week through the tail vein.All rats were killed in the 12th week.Immuno-histochemistry was performed on renal tissue to detect Collagen Ⅳ(Col-Ⅳ),fibronectin(FN),MMP-2,-9 and TIMP-1.Results Immunohistochemistry staining indicated that expressions of MMP-2,-9 in model group decreased significantly compared to sham operated group(Pa
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Applied Clinical Pediatrics
Year:
2006
Document type:
Article